Read the Edited Video Transcript for ‘Olympus SZX10 Stereo Microscope Disassembly’

Hey, this is Chad with Munday Scientific. Today, we’re going to demonstrate how to disassemble an Olympus SZX10 stereo microscope.

This particular microscope has just been sold, and we’ll be completely disassembling it for safe shipping. We’re going to show the customer how everything comes apart so they’ll also know how to put it back together once it arrives.

You’ll need a few basic Allen wrenches for the procedure, and we’ll include the necessary tools with the microscope when it’s shipped.

Turn Off and Unplug the Microscope

Before beginning any disassembly, make sure the microscope is turned off and unplugged from its power source.

Once that’s done, we can begin removing the individual components.

Removing the Binocular Head

We’ll start with the binocular head.

Using a 3 mm Allen wrench, locate and loosen the retaining screw between the binocular head and microscope body.

Keep a hand on the head while loosening the screw. Once it’s released, carefully lift the head off the microscope and set it aside in a safe location.

Lamp Housing and Bulb

At the rear of the microscope is the lamp housing.

If you ever need to replace the bulb, the lamp assembly can be pulled out from the rear of the stand. This system uses a 6V, 30W bulb.

The microscope base also has a convenient built-in handle, which makes it easier to move the microscope around the laboratory.

Removing the Microscope Assembly From the Stand

This particular Olympus SZX10 is equipped with a dual-objective nosepiece, allowing two objectives to be installed at the same time.

Before we can completely separate the microscope body from the focus mount, we’ll need to remove the microscope assembly and then remove the objectives and dual-objective nosepiece.

First, loosen the retaining control that secures the microscope assembly to the stand.

Support the microscope with your other hand as you loosen it. The entire assembly can then be carefully removed and set aside.

Removing the Analyzer and Objectives

This particular microscope is equipped for polarized light and has an analyzer installed.

The analyzer is held in place by a knurled retaining screw. Loosen the screw and carefully remove the analyzer.

Next, remove each of the two objectives from the dual-objective nosepiece and set them aside somewhere safe.

Removing the Dual-Objective Nosepiece

Once both objectives have been removed, rotate the nosepiece off-center to gain access to its mounting screws.

You’ll need a 2.5 mm Allen wrench for this portion of the disassembly.

There are three mounting screws securing the nosepiece assembly. Loosen and remove the accessible screws.

The third screw may initially be hidden, so rotate the turret until you can access it. Once all three screws have been removed, the entire dual-objective nosepiece assembly can be lifted off.

Removing the Microscope Body From the Focus Mount

Next, we’ll separate the microscope body from the focus mount.

You’ll need a 4 mm Allen wrench for this step.

Locate the retaining screw on the right side of the focus mount. Depending on the microscope, there may be a small white cap covering this screw.

We’ve already removed the cap for this demonstration.

Loosen the retaining screw while supporting the microscope body. Once the screw is loose, the microscope body can slide off the focus mount using its dovetail connection.

At this point, the main microscope assembly has been completely disassembled.

Glass Stage Plate and Polarizer

Returning to the stand, you’ll see the glass stage plate on the microscope base.

On this polarized-light configuration, the polarizer is located underneath the glass plate.

When the microscope is assembled for polarized-light observation, the analyzer can be rotated in relation to the polarizer to cross the polarizers and achieve the desired polarized-light effect.

Removing the Column

If necessary, the microscope’s vertical column can also be removed from the base.

For this procedure, you’ll need a 4 mm Allen wrench.

Loosen the two mounting screws that secure the column to the base. Once they’re loose, the column can be lifted out.

Olympus offers columns in a variety of lengths, allowing the microscope to be configured with additional working distance when required for larger specimens.

Storing the Allen Wrench

Many of these microscope columns also have a convenient storage location at the top for the 3 mm Allen wrench.

We’ll place the wrench there so the customer has easy access to it when the microscope arrives.

Reassembling the Olympus SZX10

To reassemble the microscope, simply perform the disassembly procedure in reverse order.

In general, you’ll:

1. Install and secure the column if it was removed.
2. Slide the microscope body back onto the focus mount and secure it.
3. Reinstall the dual-objective nosepiece.
4. Reinstall both objectives.
5. Reinstall the analyzer.
6. Mount the microscope assembly back onto the stand.
7. Reinstall the binocular head.
8. Verify that all components are properly seated and securely tightened.

Take your time during reassembly, particularly when handling the objectives and optical components.

Final Thoughts

That’s how you disassemble an Olympus SZX10 stereo microscope for safe transportation or shipping.

The microscope breaks down into several major components, and most of the procedure can be completed with 2.5 mm, 3 mm, and 4 mm Allen wrenches.

When putting everything back together, simply follow the same procedure in reverse.

Contact Information

If you have any questions about disassembling or reassembling your Olympus SZX10, contact Munday Scientific.

Email: info@mundaymicroscope.com
Phone: 919-775-1517

Contact Us

Online Shop: microscopemarketplace.com

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Thanks for watching!

The post Olympus SZX10 Stereo Microscope Disassembly appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Where does the blue filter go on a microscope?’

Hey, this is Chad with Munday Scientific. Today, we’re going to talk about the didymium blue filter, what it does, and where it should be installed on a microscope with halogen illumination.

For this demonstration, we’re using an Olympus BX41 microscope.

What Does the Blue Filter Do?

When you receive a microscope from us, you may find a blue filter included in a packet with the microscope. If you already have your microscope, look for a small piece of glass that may have a slightly mirrored appearance.

Halogen bulbs naturally produce light with a warmer color temperature, which can give the field of view a yellow or orange appearance.

The blue filter helps compensate for this warmer illumination, producing a cooler, whiter light through the microscope.

Installing the Filter on an Olympus BX41

On the Olympus BX41, the blue filter is positioned directly above the dust glass beneath the condenser.

When the filter is placed in the light path, you’ll immediately notice the difference in color temperature. The warmer halogen illumination changes to a cooler, whiter appearance.

Filter Location on Other Microscopes

The exact location of the blue filter will vary depending on the microscope model.

In most cases, the filter will be located somewhere underneath the condenser. Depending on the microscope, it may:

• Rest directly on top of the dust glass
• Fit into a filter holder beneath the condenser
• Insert into the bottom of the condenser assembly

Regardless of the exact mounting method, the purpose is the same: placing the blue filter in the illumination path helps correct the warmer color produced by the halogen bulb.

Questions About Your Microscope?

If you’re unsure where the didymium or blue filter belongs on your particular microscope model, contact Munday Scientific and we’ll be happy to help.

Email: info@mundaymicroscope.com
Phone: 919-775-5596

Online Shop: microscopemarketplace.com

Subscribe to Videos: Youtube Channel

Contact Us

Thanks for watching!

The post Where does the blue filter go on a microscope? appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Olympus BX51 Fluorescence DIC Breakdown’

Hey, this is Chad with Munday Scientific. Today, we’re going to take a look at this Olympus BX51 fluorescence and DIC microscope.

DIC stands for Differential Interference Contrast, also commonly referred to as Nomarski microscopy. This particular microscope has also been configured for fluorescence to meet the customer’s specific application requirements.

Let’s go through the system and take a closer look at how everything works.

Trinocular Head and Camera Light Path

The customer requested a trinocular head so they can capture fluorescence images with a camera.

The trinocular head has a pull rod on the side that controls how the microscope’s light is distributed between the eyepieces and camera.

There are three positions:

• All the way in: 100% of the light goes to the eyepieces.
• Middle position: 50% of the light goes to the eyepieces and 50% goes to the camera.
• All the way out: 100% of the light goes to the camera.

A diagram on the microscope head indicates the three positions.

Accessing the Fluorescence Filter Cubes

Next, let’s look at the fluorescence filter cubes.

To access the cubes, remove the microscope’s 3 mm Allen wrench and locate the retaining screw on the side of the fluorescence illuminator.

Loosen the screw and slide the filter cube assembly out.

This particular microscope is equipped with two fluorescence filter cubes.

Removing and Installing Filter Cubes

Each fluorescence cube is mounted on a dovetail.

To remove a cube, loosen its retaining screw and slide the cube out of the dovetail mount.

To reinstall it, slide the cube back into the dovetail and secure the retaining screw.

When using the microscope for brightfield, make sure the fluorescence filter cubes are moved out of the optical path. Otherwise, you’ll be viewing the brightfield image through the fluorescence filter.

Activating Fluorescence

To use fluorescence, the appropriate filter cube must be positioned in the light path and the fluorescence shutter must be open.

The shutter control allows you to quickly open or block the fluorescence illumination.

If you’re going to step away from the microscope while leaving the fluorescence system powered on, you can close the shutter to block the illumination until you’re ready to continue working.

LED Fluorescence Illumination

This system is also equipped with a single-channel LED fluorescence illuminator.

With the LED system, you can turn the fluorescence excitation illumination on and off directly with the illuminator control.

Turning the LED off effectively serves the same purpose as closing the fluorescence shutter when you don’t need excitation light reaching the specimen.

Understanding the DIC System

This microscope is also configured for Differential Interference Contrast (DIC).

For DIC to operate correctly, the appropriate optical components must be aligned in the light path.

The system uses an analyzer along with the corresponding DIC prism. The DIC adjustment can then be rotated to change the contrast of the image.

Objective Configuration

This BX51 is equipped with the following objectives:

• 10x
• 20x
• 40x
• 60x
• 100x

The nosepiece can be removed using the same 3 mm Allen wrench.

Locate the retaining screw beside the nosepiece and loosen it approximately a quarter turn. Once loosened, the nosepiece can slide out of the microscope.

Matching the Objective and DIC Prism

Each DIC objective must correspond with the appropriate DIC prism in the condenser.

For example, when using the 10x objective, the system needs to have:

1. The 10x objective positioned in the optical path.
2. The DIC analyzer inserted.
3. The corresponding 10x DIC prism positioned in the condenser.

All three components must be properly aligned for the DIC system to work as intended.

The same principle applies when using the 20x, 40x, and 100x DIC objectives.

Accessing the DIC Prisms

This microscope has a right-handed mechanical stage.

The stage can be removed by loosening its thumbscrew, although we’re going to leave it installed for this demonstration.

To access the condenser, lower it using the substage adjustment. Depending on the position of the stage, you may need to raise the stage slightly to provide enough clearance to remove the condenser.

Loosen the condenser retaining thumbscrew, then use the 3 mm Allen wrench as needed to access the prism assembly.

Inside, you’ll see the individual DIC prisms corresponding to the microscope’s objectives.

For example:

• 10x objective → 10x DIC prism
• 20x objective → 20x DIC prism
• 40x objective → 40x DIC prism
• 100x objective → 100x DIC prism

Using the 60x Objective With This Configuration

This particular customer requested a 60x objective, but did not request a dedicated 60x DIC prism.

However, we found that the 60x objective produces a good image when used with the 100x DIC prism in this particular setup.

This gives the customer an additional option if they want to obtain a DIC-style image while using the 60x objective.

Brightfield Illumination Controls

The Olympus BX51 also provides several controls for conventional brightfield microscopy.

The light intensity knob controls the brightness of the transmitted illumination.

The microscope is also equipped with neutral density and light-balancing filters that can be moved in and out of the light path as needed.

LBD Daylight Blue Filter

One of the most commonly used filters is the LBD daylight blue filter.

With halogen illumination, the light naturally has a warmer color temperature. The LBD filter helps compensate for this and provides a cooler, more neutral-looking illumination through the microscope.

If the brightfield image suddenly looks unusually warm or yellowish, check the LBD filter. It may be missing or simply not pushed into the optical path.

On the BX51, these filters can be easily clicked in and out as needed.

Final Thoughts

That’s the basic overview of this Olympus BX51 fluorescence and DIC microscope.

This configuration combines several imaging capabilities in a single microscope, including:

• Brightfield microscopy
• Fluorescence microscopy
• Differential Interference Contrast (DIC)
• Trinocular imaging for camera use
• Multiple objective magnifications from 10x through 100x

The key with DIC is making sure the objective, analyzer, and corresponding DIC prism are properly aligned. For fluorescence, make sure the appropriate filter cube is in the light path and the fluorescence illumination is activated.

Contact Information

If you have any questions about an Olympus BX51, fluorescence microscopy, DIC configurations, or other microscope equipment, feel free to contact Munday Scientific.

Email: info@mundaymicroscope.com

Online Shop: microscopemarketplace.com

Contact Info

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The post Olympus BX51 Fluorescence DIC Breakdown appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Olympus BX45 Basics Demonstration’

Hey, this is Chad with Munday Scientific. Today, we’re going to take a closer look at the Olympus BX45 microscope.

The BX45 is a very popular microscope, and we’ve had quite a few of them going out the door recently. We’re going to walk through the configuration of this particular system and show you a few quick tips that may help you get the most out of your microscope.

Built-In 3 mm Allen Wrench

One convenient feature of the Olympus BX Series microscopes is the built-in 3 mm Allen wrench.

The wrench is stored on the microscope stand and can be used for most of the adjustments and procedures we’ll demonstrate. If the original wrench is missing from your microscope, any standard 3 mm Allen wrench should work.

Microscope Head Options

This particular BX45 is currently equipped with a trinocular head, but there are several head configurations available for this microscope.

Depending on your application, you could use:

• A standard fixed binocular head
• A trinocular head
• A black tilting head
• A tilting and telescoping ergonomic head

The customer receiving this particular microscope selected the tilting and telescoping head, so we’ll install that configuration.

The head locks onto the microscope using the retaining screw. Once installed, the telescoping design allows you to move the eyepieces forward or backward to create a more comfortable viewing position.

This can be particularly useful when you want to sit farther back from your desk or workstation.

Fixed Nosepiece Design

On the Olympus BX45, the nosepiece is fixed and travels vertically as you adjust the microscope’s focus.

The nosepiece also includes a dust plug that can be removed if you need to install an analyzer.

The BX45 can be configured with either a five-position or six-position nosepiece. If you need to convert to a six-position nosepiece, we recommend having an authorized microscope service technician perform the change.

Removing the Stage

Before removing the stage, raise the nosepiece completely and make sure the objectives are safely out of the way.

This is important because you don’t want to accidentally hit or damage an objective while lifting the stage off the microscope.

Once you have enough clearance, carefully remove the stage and set it aside.

Condenser Top Lens

With the stage removed, we can get a better look at the condenser system.

The condenser has a top lens that swings in and out of the optical path.

When using a 2x or 1.25x objective, swing the top lens out to properly fill the field of view.

When you move to the 10x objective or higher, swing the top lens back into position.

Setting Köhler Illumination

The 10x objective is typically used when setting Köhler illumination.

The condenser has two centering controls that allow you to properly center the illumination system.

The centering keys are removable and can be threaded into the corresponding adjustment holes when needed.

We have a separate video showing the complete procedure for setting Köhler illumination on this microscope, and we’ll include a link in the video description.

Adding Phase Contrast or a Blue Filter

The condenser assembly can also be configured for additional applications.

Using the retaining screw, you can remove the condenser cassette and access the internal positions for accessories.

Depending on your application, you can install:

• Phase rings
• A daylight blue filter

Adding the appropriate phase rings allows the BX45 to be configured for phase contrast microscopy.

The daylight blue filter can be used with a halogen illumination system to reduce the naturally warm appearance of the halogen light and provide a cooler-looking field.

Adding a Polarizer and Analyzer

The BX45 can also be equipped with a polarizer and analyzer.

The polarizer installs beneath the condenser, while the analyzer is inserted into the analyzer slot near the nosepiece.

With both components installed, you can cross the polarizers for applications requiring polarized illumination.

Repositioning the Fine Focus Control

Here’s another useful adjustment that can improve the ergonomics of the microscope.

On this BX45, the extended fine focus knob is positioned on the right side. Depending on your stage configuration, this knob can interfere with the stage control.

Fortunately, the extended fine focus control can be moved to the opposite side.

First, remove the rubber cover from the focus knob. You’ll find a retaining screw underneath.

Using the same 3 mm Allen wrench, loosen the screw and remove the extended fine focus knob.

On the opposite side, there may be a small cover or sticker over the mounting hole. Carefully remove it using your fingernail, a small flathead screwdriver, or another suitable tool.

Move the extended fine focus control to that side and secure it in place. The cover can then be transferred to the unused hole on the opposite side.

Once the stage is reinstalled, you’ll notice that the stage control is much easier to operate because the extended fine focus knob is no longer interfering with it.

Light and Field Diaphragm Controls

The microscope’s illumination intensity control allows you to adjust the brightness of the light.

The field diaphragm is also located on the microscope base and is used when setting Köhler illumination.

Together with the condenser controls, these adjustments allow you to properly configure the microscope’s illumination for your specimen.

Changing the Halogen Bulb

We have another video that goes into more detail about changing the bulb, but the procedure is straightforward.

Before replacing the bulb, always turn the microscope off and disconnect it from power.

The BX45 shown here uses a 6V, 30W halogen bulb located in the lamp housing at the rear of the microscope.

Remove the lamp housing, replace the bulb, and reinstall the assembly.

Many BX Series microscopes are now being upgraded to LED illumination, but the original halogen system is still a good option. Replacement bulbs and lamp components are readily available and relatively inexpensive.

Why the Olympus BX45 Is Popular

The Olympus BX45 is an excellent microscope, particularly for pathology and cytology applications.

One of its biggest advantages is ergonomics. The low-positioned controls allow the user to rest their hands comfortably on the work surface while operating the stage and focus controls.

Combined with an ergonomic tilting and telescoping head, this makes the BX45 particularly comfortable for professionals who spend extended periods working at the microscope.

Final Thoughts

The Olympus BX45 is a versatile microscope with a wide range of configuration options.

Depending on your application, it can be equipped with different viewing heads, five- or six-position nosepieces, low-power objectives, phase contrast components, polarized illumination, and either halogen or LED lighting.

Its ergonomic design and flexible configuration make it particularly well suited for pathology, cytology, and other laboratory applications.

Contact Information

If you have any questions about the Olympus BX45 or are interested in purchasing a refurbished Olympus BX Series microscope, contact Munday Scientific.

Email: info@mundaymicroscope.com

Contact Us

Online Shop: microscopemarketplace.com

Subscribe to videos: Youtube Channel

Thanks for watching!

The post Olympus BX45 Basics Demonstration appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘BX43 or BX46 Light is Blinking’

Hey, this is Chad with Munday Scientific. Today, we’re going to talk about what to do when the LED light starts blinking or strobing on an Olympus BX43 or BX46 microscope.

For this demonstration, we’re using an Olympus BX46.

Why the LED May Start Blinking

Occasionally, the LED illuminator on an Olympus BX43 or BX46 may begin to strobe or blink.

This can happen after the microscope has been left on for extended periods or simply as the LED assembly gets older and approaches the end of its service life. You may begin to see this issue on systems that are around 10 years old or older.

A blinking or strobing light can make it extremely difficult to view and read a specimen, so when this occurs, the LED assembly will typically need to be replaced.

We’ll include information about the correct replacement part in the video description.

Preparing the Microscope

Before replacing the LED, turn the microscope off and unplug it from the power source.

You’ll need a 3 mm Allen wrench for the replacement procedure. Olympus typically includes this wrench with the microscope, and it can usually be found stored on the back of the stand.

Disconnecting the LED

Turn the microscope around so you can access the rear of the stand.

Locate the electrical connection for the LED assembly and unplug it from the microscope.

Removing the LED Assembly

On the right side of the microscope, locate the small access hole for the LED assembly’s retaining screw.

Insert the 3 mm Allen wrench into the hole and loosen the screw.

Once the retaining screw has been loosened, the LED assembly will slide out of the microscope.

Installing the Replacement LED

Take the new LED assembly and slide it into the same opening.

Once it is fully seated:

1. Tighten the retaining screw with the 3 mm Allen wrench.
2. Reconnect the LED’s electrical plug.
3. Plug the microscope back into its power source.
4. Turn the microscope on and verify that the illumination is operating properly.

Your Olympus BX43 or BX46 should now have steady illumination again without the distracting blinking or strobing.

Final Thoughts

If the LED illumination on your Olympus BX43 or BX46 begins blinking or strobing, it may be an indication that the LED assembly is reaching the end of its service life.

Fortunately, replacing the assembly is a relatively simple procedure requiring only a 3 mm Allen wrench and the correct replacement LED.

Contact Information

If you have any questions about replacing the LED on your Olympus BX43 or BX46 microscope, please give us a call.

Phone: 919-775-1517

Contact Information

Online Shop: microscopemarketplace.com

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Thanks for watching!

The post BX43 or BX46 Light is Blinking appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Assemble and Disassemble an Olympus CK2’

Hey, this is Chad with Munday Scientific. Today, we’re going to talk about the Olympus CK2 microscope and show you how to assemble and disassemble it.

When you receive this microscope from us, it will be shipped partially disassembled to help protect the components during transit. We’ll go through the major pieces and show you how everything fits back together.

Removing and Installing the Specimen Holder

If your microscope includes a slide holder or specimen holder, it will typically be removed for shipping.

The holder is secured by two knurled screws located underneath the stage.

To remove the holder, simply loosen the two screws. They’re normally only finger-tight, but each screw also has a slot that allows you to use a flathead screwdriver if necessary.

Once both screws are loosened, the specimen holder will lift off the stage.

Installation is simply the reverse process. Position the holder correctly on the stage and tighten the two screws until they are secure.

Removing and Installing the Microscope Head

The microscope head is secured by a thumbscrew and flange.

To install the head, position the flange underneath the two retaining prongs and tighten the thumbscrew.

Always keep one hand on the microscope head while tightening it to prevent it from slipping or falling.

Once the thumbscrew is secure, gently wiggle the head to verify that it is firmly attached.

Installing the Phase Slider

The phase slider will also be removed when the microscope is shipped.

Depending on the microscope’s configuration, the slider will typically include positions corresponding to the objectives being used. On this particular microscope, we have 10x and 20x phase objectives, along with a position for the 4x objective.

The phase slider aligns the appropriate phase rings with the corresponding objectives.

Make sure the correct phase position is selected for the objective you’re using.

Installing the Filter

The microscope also has a position for a colored filter.

Depending on the configuration, this may include:

• A daylight blue filter
• A clear or blank filter
• A green/yellow filter, as shown on this microscope

The filter simply installs in its designated holder in the illumination system.

Removing the Illuminator Column

The illuminator column is another component that we always remove before shipping the microscope.

Before removing the column, locate the electrical connection on the back. This socket connection will be unplugged when the column is removed.

When reinstalling the column, remember to reconnect this plug so the microscope’s illumination system will operate.

Removing the Column From the Microscope

For this procedure, you’ll need a 3 mm Allen wrench.

Before tilting or moving the microscope, it’s a good idea to remove any loose filter from the illuminator so it doesn’t fall out.

Locate the two mounting screws that secure the column to the microscope.

Using the 3 mm Allen wrench, loosen and remove these screws while keeping your other hand firmly on the column for support.

Once the screws have been removed, the column can be lifted away from the microscope.

Reinstalling and Aligning the Column

Reinstalling the illuminator column is straightforward because Olympus incorporated alignment pins into the assembly.

On the bottom of the column, you’ll see locating pins that correspond with matching holes in the microscope base.

Align the pins with these holes and lower the column into position.

Because the alignment is preset by these locating pins, the column should automatically sit in the proper position.

Once it’s seated correctly, reinstall the two mounting screws and tighten them securely with the 3 mm Allen wrench.

Finally, reconnect the electrical plug on the back of the microscope so the illumination system will function.

Final Assembly Check

Once your Olympus CK2 has been assembled, verify that:

• The specimen holder is securely attached.
• The microscope head is properly seated and tightened.
• The phase slider is installed correctly.
• The appropriate filter is in place.
• The illuminator column is securely mounted.
• The illuminator’s electrical connection has been reconnected.

Give each installed component a gentle check to make sure everything is secure before using the microscope.

Contact Information

If you have any questions about assembling or disassembling your Olympus CK2 microscope, please give us a call.

Phone: 919-775-1517

Online Shop: microscopemarketplace.com

Contact Information

Subscribe to videos: Youtube Channel

Thanks for watching!

The post Assemble and Disassemble an Olympus CK2 appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Testing Out a Leica MZ16 Stereo Microscope’

Good morning. This is Chad with Munday Scientific. Today, we’re going to use this Leica MZ16 stereo microscope to show you how to make sure everything remains properly parfocal throughout the zoom range.

A common issue with zoom stereo microscopes is losing focus as you increase or decrease magnification. Fortunately, checking and adjusting parfocality is a fairly simple procedure.

When I first started working with microscopes, this was something I struggled with, so we wanted to put together a quick video showing you how to do it correctly.

Bring the Specimen Into Focus

The first step is to place a specimen under the microscope and bring it into focus.

For this demonstration, we’re simply going to use an envelope as our specimen.

Secure the specimen on the base, then use the microscope’s focus control to bring the image into sharp focus.

Check the Eyepiece Diopter Settings

Next, check the diopter adjustment on each eyepiece.

The diopter adjustments allow you to compensate for differences in your eyesight. Each eyepiece has a reference point and a zero setting.

If you have 20/20 vision, you’ll typically want both eyepieces set to zero. Depending on the eyepiece, you may see a line indicating the zero position, along with positive and negative adjustment markings.

Make sure both eyepieces are set equally at zero before proceeding.

If your vision requires correction, you may need to adjust the diopters accordingly rather than leaving them at zero.

Focus at the Highest Magnification

Once the eyepieces are properly adjusted, zoom the microscope all the way in to its highest magnification.

At maximum magnification, use the microscope’s focus control to bring the specimen into sharp focus.

It’s important to establish your primary focus at the highest magnification because this provides the most precise point for setting and checking parfocality.

Check Focus Throughout the Zoom Range

Once the specimen is sharply focused at maximum magnification, slowly zoom the microscope back out.

The specimen should remain in focus throughout the entire zoom range.

You can then zoom back in and verify that the image continues to remain sharp without requiring significant refocusing.

If the image remains in focus as you move from low to high magnification and back again, the microscope is properly parfocal.

What If the Microscope Doesn’t Stay in Focus?

If you’re losing focus as you move through the zoom range, there may be another issue that needs to be addressed.

Possible causes can include:

• Incorrectly adjusted eyepiece diopters
• The front lens being used with the microscope
• Vision correction required for the individual user
• Another component in the optical system affecting parfocality

Checking the eyepiece settings and making sure the microscope is properly configured can often help identify the source of the problem.

Final Thoughts

This particular Leica MZ16 is perfectly parfocal and is getting ready to go out the door to its new owner.

Once you know the proper procedure, checking parfocality on a zoom stereo microscope is quick and straightforward:

1. Place your specimen under the microscope.
2. Verify the eyepiece diopter settings.
3. Zoom to maximum magnification.
4. Bring the specimen into sharp focus.
5. Zoom through the entire magnification range.
6. Confirm that the specimen remains in focus.

Contact Information

If you’re looking for used laboratory equipment or refurbished microscopes, contact us.

Phone: 919-775-5596
Online Shop: microscopemarketplace.com
Subscribe to Videos: Youtube Channel

Thanks for watching!

The post Testing Out a Leica MZ16 Stereo Microscope appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Olympus BX45 Pathology Microscope’

Hey, this is Chad with Munday Scientific. Today, we’re going to take a look at the Olympus BX45 microscope.

The BX45 is a great microscope for a variety of applications, but the system we’re showing today is configured specifically for pathology.

Ergonomic Tilting Head

This microscope is equipped with an Olympus U-TBI-3 ergonomic tilting binocular head.

This is an excellent head for pathology applications because the viewing angle can be adjusted to provide a more comfortable working position. You can sit back while viewing specimens, helping reduce neck strain during extended periods of microscope use.

Other heads can also be installed on the BX45, including a standard fixed binocular head or a trinocular head, but the ergonomic tilting head is one of our favorite configurations. It’s comfortable, durable, and very well suited for everyday pathology work.

Wide-Field Eyepieces

This microscope is equipped with Olympus 10x eyepieces featuring a wide 22 mm field of view.

The wider field allows you to see more of the specimen at once and provides a comfortable viewing experience.

Objective Configuration

For this pathology setup, we’ve installed five objectives:

• 2x
• 4x
• 10x
• 20x
• 40x

This is probably our most popular objective configuration for pathology applications and provides a useful range of magnifications for examining specimens.

Adding an Analyzer and Polarizer

The BX45 also provides the ability to add an analyzer and polarizer when needed.

There’s a dedicated slot on the microscope for the analyzer, while the polarizer can be installed in the appropriate slot below the condenser.

We have another video demonstrating how to install and use these components, which we’ll link in the video description.

Condenser and Köhler Illumination Controls

The condenser has two centering controls that allow you to properly center the condenser when setting Köhler illumination.

We’ve also produced a separate video showing how to set Köhler illumination, and we’ll include a link to that tutorial in the description.

The microscope includes a control for raising and lowering the condenser, as well as a separate control for swinging the condenser’s top lens element in and out of the optical path.

Built-In Allen Wrench

One convenient feature of the Olympus BX Series is the included Allen wrench.

The wrench is stored directly on the microscope in the back-right corner, so it’s readily available whenever you need to make adjustments or remove certain components.

Changing the Condenser Cassette

If you’re using the microscope for an application other than pathology, such as phase contrast, the condenser can be reconfigured.

Using the supplied Allen wrench, loosen the retaining screw near the condenser assembly and slide the cassette out.

This allows you to install the appropriate phase rings for phase contrast applications.

Daylight Blue Filter

This BX45 is also equipped with a daylight blue filter.

Because a halogen bulb naturally produces warmer, more orange-colored illumination, the daylight blue filter helps provide a cooler, whiter appearance.

The halogen illumination system can also be upgraded to LED illumination if desired, giving the microscope a more modern lighting system.

Changing the Bulb

If you need to replace the bulb, first turn the microscope off and unplug it from the power source.

The lamp housing is located at the rear of the microscope and contains a 6V, 30W bulb.

To access the bulb, squeeze the lamp housing cover and gently work it free. You can then remove and replace the bulb before reinstalling the cover.

We have a separate video demonstrating the complete bulb replacement procedure, which we’ll also link in the description.

Final Thoughts

The Olympus BX45 is a versatile and durable microscope that can be configured for a wide range of laboratory applications.

For pathology, the combination of an ergonomic tilting head, wide-field 10x eyepieces, and 2x, 4x, 10x, 20x, and 40x objectives provides an excellent setup for comfortable everyday use.

Contact Information

If you’re looking for an Olympus BX45 or another refurbished Olympus BX Series microscope, we’re happy to help.

Visit our online shop microscopemarketplace.com for available microscopes, accessories, and other products.

Thanks for watching!

Contact us

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The post Olympus BX45 Pathology Microscope appeared first on Munday Scientific Instruments.

Read the Edited Video Transcript for ‘Where does the Blue Filter go?’

Hey, this is Chad with Munday Scientific. Today, we’re going to talk about the didymium blue filter and where it should be installed on a microscope with halogen illumination.

For this demonstration, we’re using an Olympus BX41 microscope.

Purpose of the Blue Filter

When you receive a microscope from us, you may find this filter included in a packet with the microscope. If you already have your microscope, look for a small piece of glass that may have a slightly mirrored appearance.

A halogen bulb naturally produces light with a warmer color temperature. The purpose of the blue filter is to alter the appearance of that illumination, producing a cooler, whiter light through the microscope.

Installing the Filter on an Olympus BX41

On the Olympus BX41, the blue filter is placed directly above the dust glass beneath the condenser.

When the filter is inserted, you’ll immediately notice a change in the color of the illumination. The warmer halogen light takes on a cooler, whiter appearance.

Filter Location on Other Microscopes

The exact location of the blue filter can vary depending on the microscope model.

In most cases, you’ll find the filter positioned underneath the condenser. Depending on the microscope, it may:

• Rest directly on the dust glass
• Insert into a filter holder beneath the condenser
• Fit into the bottom portion of the condenser assembly

If you’re unsure where the didymium or blue filter belongs on your particular microscope, feel free to contact us and we’ll be happy to help.

Contact Information

If you have questions about your microscope or the proper placement of its blue filter, contact Munday Scientific.

Email: info@mundaymicroscope.com
Phone: 919-775-5596

Online Shop: microscopemarketplace.com

Contact Us

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Read the Edited Video Transcript for ‘Plug in an Olympus BX43 or BX46’

Today, we’re going to show you how to properly connect the power supply on an Olympus BX43 or BX46 microscope.

We recently received a call about this, and the setup can be a little confusing because both the microscope and certain accessories use similar-looking power connections.

Connecting the Nosepiece

First, turn the microscope around so you can access the connections on the back.

The cable from the nosepiece feeds through the opening in the rear of the microscope. When connecting it, you’ll notice an arrow on the connector and a corresponding arrow on the microscope connection.

Line up these two arrows and plug the nosepiece into the microscope.

Connecting the Microscope Power Supply

The microscope itself uses a 5V power supply. This is very important because some Olympus power supplies use the same style of connector even though they provide different voltages.

Before connecting anything, make sure the microscope is turned off.

Plug the 5V power supply into the appropriate power connection on the microscope. Then connect a standard line cord to the power supply and plug it into a wall outlet.

Once everything is properly connected, you can turn the microscope on.

Pay Close Attention to the Power Supply Voltage

It’s extremely important to use the correct power supply.

For example, we have a 15V power supply that uses the same type of connector as the microscope’s 5V power supply. However, this 15V power supply is intended for a discussion bridge, not the microscope itself.

The 15V adapter would plug into the discussion bridge’s power connection.

Do not plug the 15V power supply into the microscope. If you connect the wrong power supply and turn the system on, you can damage the electronics.

Check the Labels Before Connecting Power

When we ship these systems, we label the power supplies to make it easy to identify the correct one.

The discussion bridge or pointer power supply will have a large white label identifying its purpose. Likewise, the microscope’s power adapter will be clearly labeled as the Olympus BX power adapter for the microscope.

Always check these labels and verify that you’re using the correct power supply before turning on the system.

Questions

If you have any questions about connecting your Olympus BX43 or BX46 microscope, give us a call at 919-775-5596 or see the following contact methods for other resources.

Online Shop: microscopemarketplace.com

Contact Us

Subscribe: Youtube Channel

Good luck, and thanks for watching.

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