Olympus BHS DIC & Fluorescence Setup
Read the Edited Video Transcript for ‘Olympus BHS DIC & Fluorescence Setup’
Hey, this is Chad with Munday Scientific. Today we’re going to talk about an Olympus BHS microscope that has been upgraded with an LED light source and outfitted with both fluorescence and DIC (Differential Interference Contrast).
Michael is going to come in and help walk through the setup and features of this microscope.
Trinocular Head and Eyepieces
This microscope is equipped with a trinocular head, allowing for both viewing and imaging.
Features
- High-point 10× eyepieces
- Diopter adjustment on one side for fine focus
- Trinocular port for adding a camera
Light Path Selection
The trinocular head includes a light path selector:
- All the way in: 100% of light to the eyepieces
- Middle position: Light split between eyepieces and camera
- All the way out: 100% of light to the camera
There is also a thumb screw on the back used to remove or secure the head.
Analyzer and DIC Components
Because this microscope is configured for DIC, it includes an analyzer.
Analyzer Positions
- Out position: Analyzer removed
- In position: Analyzer engaged
Each position has a click stop for precise placement.
Fluorescence Filter Cubes
This microscope includes a fluorescence filter cube turret.
Available Filters
- Green filter
- Blue filter
- Brightfield position
You can rotate the turret to select the desired filter for your application.
DIC Prism System
The microscope is equipped with a DIC prism system.
- The prism slider inserts into position and can be locked with a screw
- The system is configured with SPL (SPLAN) optics, including phase-compatible objectives
Objectives and Phase Compatibility
This microscope uses SPLAN objectives, including phase-capable optics.
For proper phase contrast:
- The phase ring in the condenser must match the objective in use
- This may involve either:
- A phase contrast condenser, or
- Phase prisms installed in the condenser
In this setup, the microscope has been configured primarily for DIC testing and operation.
Removing the Stage and Nosepiece
To access internal components:
- Lower the stage
- Remove the right-handed stage
- If needed, remove the nosepiece using the thumb screw
The nosepiece will slide out for easier access.
Condenser and Prism Assembly
The condenser is adjustable using the height adjustment knob and can be removed using a locking mechanism.
Features of the Condenser
- Flip-top lens for different magnification needs
- Interchangeable prism assembly, including:
- Brightfield stop
- 10× prism
- 20× prism
- 40× prism
- Darkfield stop
Diaphragm
The condenser includes an aperture diaphragm that adjusts contrast.
Polarizer and DIC Setup
The condenser also includes a polarizer, which can be:
- Rotated freely
- Locked into position using a dial
Basic DIC Alignment Process
- Remove the prism
- Insert the analyzer
- Rotate the polarizer in brightfield mode
- Adjust until the field goes completely dark (extinction)
- Insert the appropriate prism (e.g., 10× prism)
- Match it with the corresponding objective
At this point, DIC imaging can be achieved.
Reinstalling the Condenser
When reinstalling the condenser:
- Align the guide pin with the slot
- Slide it into place
- Lock it securely
LED Upgrade
This microscope was originally a 12V 100W halogen system, but it has been upgraded to a Nanodyne LED light source.
Benefits of LED Upgrade
- Brighter illumination
- Longer lifespan
- Eliminates issues with aging halogen electronics (often 30–40 years old)
Mechanical Refurbishment
This microscope has been fully serviced, including:
- Complete relubrication
- New gearbox
- Rebuilt coarse and fine focus system
It is now fully restored and ready for use.
Fluorescence System Overview
The microscope includes a fluorescence illumination system.
Key Components
- Shutter: Must be open for light to pass
- Collector lens: Adjusts light focus
- Centering keys: Used for lamp alignment
Mercury Power Supply Connection
When connecting the mercury power supply:
- Align the keyways on the connector
- The larger notch must face upward
- Insert and rotate to lock into place
If the connector is not aligned properly, it will not seat correctly.
Final Thoughts
This Olympus BHS is a fully upgraded and highly capable microscope, suitable for:
- Fluorescence microscopy
- DIC imaging
- Brightfield and darkfield applications
It combines modern LED performance with the durability of a classic Olympus platform.
Where to Buy & Contact Information
If you have questions about this microscope or others like it, feel free to reach out.
Website: microscopemarketplace.com
Email:
Phone: 919-775-5596
Closing
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