Nikon E400 Microscope Plus Nanodyne LED

Read the Edited Video Transcript for ‘Nikon E400 Microscope Plus Nanodyne LED’

Hi, this is Chad with Munday Scientific. Today, we’re going to talk about the Nikon E400 microscope. Mike’s going to come in and help us out. This is a great clinical microscope, and we’ll be working from the top down.

Head & Camera Port

This particular unit features a tilting, telescoping head—a newer style also used on models like the
Nikon Eclipse CI and 50i series. It works perfectly with the E400, and this head was specifically requested by the customer.

It includes a CEP, which is the camera port. It can be removed with a 2 mm Allen wrench by inserting it into this hole. The piece pulls right out. If the CEP feels loose, be sure that screw is tightened.

To send light to the camera, you pull this knob here. The scope includes 10x, 22 mm field-of-view eyepieces, both of which are focusable.

Adding a Camera Port (Alternative)

If you don’t already have this head and still want to add a camera, you would need a part called the YDP.
We keep all components bagged here for protection.

This YDP port splits the light either 100% to the eyepieces or in a 55/45% split between eyepieces and camera. To install:

• Remove the thumbscrew
• Take out these white plastic caps on either side
  • These caps can sometimes prevent the piece from seating properly, causing uneven imaging
• Install the unit and tighten it down against the flange
  • It can technically work without screws, but we do recommend using them for stability

Nosepiece & Objectives

This microscope has a quintuple nosepiece loaded with:

• Plan 4x
• Fluorite 10x, 20x, and 40x objectives
  (This setup was customized per the customer’s request)

There’s an empty nosepiece slot currently covered with a dust cap. You could optionally add a 2x objective for pathology or a flip-out condenser depending on your application.

Condenser, Stage & Illumination

The scope includes:

• A standard Abbe condenser
• A right-handed coaxial mechanical stage with a smooth-operating slide holder

To remove the condenser:

• Lower the substage using this knob
• Loosen this thumbscrew and slide the condenser out
  It features a working iris diaphragm for controlling contrast.

There’s also a field diaphragm under the condenser for setting Köhler illumination.

LED Upgrade

This E400 has been upgraded to an LED illumination system using a Nanodyne LED module. We also replaced the rheostat.

• The new rheostat features click-stop control and produces consistent, even light.
• With this LED upgrade, you no longer need a blue filter or ND (neutral density) filters.

E400 microscopes are a bit older, and the original halogen rheostats tend to fail over time—causing flickering or dead zones when adjusting the light. If your halogen microscope is showing these symptoms, it may be time to upgrade to LED or replace the rheostat.

We do all wiring internally so you can still use the original line cord. While some companies recommend external plug-and-play installation, we prefer the cleaner, more permanent internal wiring solution.

Focus & Tension Adjustment

The scope features:

• Inner tension adjustment knob
• Coarse focus (outer knob)
• Fine focus (inner knob)

If you notice the stage drifting on its own during focusing, the tension knob likely needs tightening.

Final Thoughts ‘Nikon E400 Microscope Plus Nanodyne LED’

This is an excellent clinical microscope, and the Nanodyne LED upgrade is a great way to breathe new life into an older model—especially if you’re on a budget or looking for a refurbished alternative to buying new.

We completely rebuild these units and replace outdated electronics, so with this upgrade, you’re essentially getting a microscope that performs like new.

These objectives are still compatible with current Nikon CI models, so parts and upgrades remain readily available.

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